Cryo-ET white paper
Whitepaper

The rise of cryo-ET: its applications, advancements, and future


 

What will you learn?
  • Learn how new techniques like cryo-FIB milling and subtomogram averaging are improving resolution and expanding cryo-ET's use in cellular imaging
  • Understand the differences and complementary roles of cryo-ET and SPA EM in structural biology
  • Discover how cryo-ET is applied to study diverse biological structures, from viruses to organelles, offering deeper insights into cellular processes
  • Learn about upcoming innovations in cryo-ET and their potential to transform molecular visualization in native environments
Imaging across scales and integration of high-resolution structures into large volume data

Learn about the latest advancements, applications, and future directions of cryo-electron tomography (cryo-ET) in structural biology

Cryo-ET allows for high-resolution imaging of macromolecular complexes in their native cellular environment, overcoming limitations of traditional methods that rely on purified proteins. The white paper highlights key technological improvements in sample preparation and data processing, such as cryo-FIB milling and subtomogram averaging, which have broadened the scope of cryo-ET applications from single cells to multicellular organisms. The paper also explores the potential future developments in cryo-ET technology and methodology, suggesting that continued innovations could further enhance our understanding of cellular processes at the molecular level.

Image courtesy - Turk, M., & Baumeister, W. (2020). The promise and the challenges of cryo‐electron tomography. FEBS Letters, 594(20), 3243–3261. https://doi.org/10.1002/1873-3468.13948

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