What you learn

  • How to use METEOR for targeted cryo-focused ion beam (cryo-FIB) milling of yeast cells
  • How to confirm the presence of the region of interest (ROI) in the lamella during and after FIB milling and ensure only high quality lamellae were transferred to the transmission electron microscope (TEM)
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Guided cryo-lamellae preparation from yeast by using the integrated fluorescent light microscope METEOR

Download this application note to learn about the potential of METEOR for simplifying the cryo-ET workflow by preparing targeted cryo-lamellae from Saccharomyces cerevisiae (yeast) overexpressing Ede1 tagged with enhanced green fluorescent protein (eGFP).