What you learn

  • What are the main challenges of sample preparation for integrated CLEM
  • What are the protocols for several biological samples
  • What results you can achieve
     Thumbnail SECOM White Paper Sample preparation

 

Sample Preparation for Correlative Light and Electron Microscopy

One of the challenges associated with integrated CLEM (iCLEM) is the preparation of samples suitable for both FM and EM. In this white paper, we discuss several methods to ensure an integrated imaging workflow, and present results obtained using a variety of techniques and samples, including: cultured cells and tissue sections, chemical fixation or cryo-fixation, genetic labels and immunolabeled samples.