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Technical advances of preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

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Delmic Cryo-ET Journal Club Series (E4) - Nature protocol: Cryo-ET sample preparation from whole cells

In this Journal club, our hosts Dr. Caspar Jonker and Marit Smeets will discuss the workflow described by Wagner et al. in a protocol named “Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography”. We will also dive into the equipment and materials described in the article and discuss which technical advances might make this protocol a little easier in the future.