---
title: "Watch Journal Club (E4): Nature protocol: Cryo-ET sample preparation from whole cells  | DELMIC"
description: Our hosts Dr. Caspar Jonker and Marit Smeets will discuss the workflow described by Wagner et al. in a protocol named “Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography”, and compare It to other workflows that have been described over time.
---

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# What you learn

Technical advances of preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

![blog.delmic.comhubfsWebsiteBlogJournal Club Series_Episode 4 Banner_Blogpost](https://request.delmic.com/hs-fs/hubfs/social-suggested-images/blog.delmic.comhubfsWebsiteBlogJournal%20Club%20Series_Episode%204%20Banner_Blogpost.png?width=239&name=blog.delmic.comhubfsWebsiteBlogJournal%20Club%20Series_Episode%204%20Banner_Blogpost.png)

 

## Delmic Cryo-ET Journal Club Series (E4) - Nature protocol: Cryo-ET sample preparation from whole cells

In this Journal club, our hosts Dr. Caspar Jonker and Marit Smeets will discuss the workflow described by Wagner et al. in a protocol named “Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography”. We will also dive into the equipment and materials described in the article and discuss which technical advances might make this protocol a little easier in the future.

[![DELMIC](https://request.delmic.com/hubfs/Delmic_Black+Blue(preferred).svg "DELMIC")](https://www.delmic.com?hsLang=en)