Discover which factors in your cryo-CLEM workflow affect your cryo-FLM image quality, and how you can use this knowledge to optimize your image quality.
The use of cryo-fluorescent light microscopy (cryo-FLM) as part of the cryo-electron tomography (cryo-ET) workflow is on the rise. This is because cryo-FLM plays a crucial role in accurately locating the macromolecules of interest within the large cryo sample during lamella milling. However, acquiring images with a high enough resolution for accurate milling poses a challenge. Download this whitepaper as a short guide on understanding the various factors affecting cryo-FLM image quality and optimizing your overall image quality.
Images courtesy of Dr. S. Berkap, Forschungszentrum Jülich, Germany.
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