What you'll learn

  • Make cryo-ET workflows more efficient and accessible, from preparation to lamella production.

  • Precise targeting and preservation to capture native biological structures.

  • Simplify complex workflows and improve reproducibility.

Delmic Cryo-ET Online Symposium 2025

Watch the sessions from our online symposium that encompasses talks and panel discussions. Discover the latest innovations in cryo-electron tomography (cryo-ET) from leading researchers around the world. This symposium brought together new methods, tools, and applications that are advancing structural biology at the cellular and tissue level, giving new insights into:

  • FIB-view and registration-free milling lamella targeting strategies overcoming axial resolution limitations.
  • Augmenting available milling sites with SLICK and elongating cryo stage time for high sample yields  
  • Vitrification strategies and cryo-plasma FIB to study larger, more complex tissues for in situ novel insights.

 

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Speakers

Dr. Shiwei Zhu – Improving sample preparation with SLICK
Zhu presented the Sandwich-Like Culturing Kit (SLICK), a novel method designed to increase the yield of lamellae for cryo-ET. By supporting cell growth directly on EM grids, SLICK enhances sample preservation and streamlines preparation, making it easier to capture rare biological events.

Dr. Peter Dahlberg – Registration-free guidance for cryo FIB milling
Dahlberg introduced a workflow for guiding cryo-FIB milling without the need for complex image registration. His approach surpasses the axial diffraction limit, providing researchers with more accurate targeting of regions of interest while reducing time and errors in the milling process.

Ms. Cristina Capitanio & Dr. Matthias Poge – FIB-view: seeing from the FIB’s perspective
Capitanio and Poge demonstrated FIB-view, a new technique that collects fluorescence images from the perspective of the FIB. This unique approach allows researchers to better guide milling, leading to more reliable lamella preparation and a deeper correlation between fluorescence and cryo-ET data.

Dr. Cathy Spangler – Structural insights into synapses using cryo-ET
Spangler shares research on native AMPA receptors in brain tissue, offering a window into the molecular machinery of synapses. Her work demonstrates how cryo-ET can be used to reveal the structural organization of receptors within their natural environment.

Dr. Yi-Wei Chang – Cryo-plasma FIB for human brain tissue
Chang discussed preparation of brain tissue using cryo-plasma FIB, showing how large and complex samples can be made suitable for high-resolution cryo-ET. This technique enables new insights into neuronal circuits and tissue-level architecture.

Mr. Jean Daraspe – Cryo-FIB/SEM lamellae generation as a service
Daraspe presented the opportunities of offering cryo-FIB/SEM lamella preparation as a service on shared EM platforms. This approach can make advanced cryo-ET workflows more accessible to institutions and researchers who may not have direct access to specialized infrastructure.

Watch the recordings now!